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目的观察急性心肌梗死(AMI)大鼠胃排空的变化,并探讨其可能的作用机制。方法对12只 AMI 模型大鼠(AMI 组)和9只假手术大鼠(假手术组)用锝~(99m)亚锡喷替酸~(99m)TcDTPA)标记的面粉糊灌胃后,分别在单光子发射计算机断层照相仪下进行胃排空功能检查;50min后处死 AMI 组大鼠,氯化-2,3,5-三苯基四氮唑染色法计算心肌梗死面积:同时取 AMI 组、假手术组大鼠各9只,放射免疫法测定血浆胃动素。结果 AMI 组胃半排空时间(GET_(1/2))为(23.1±4.7)min,50min 胃内核素残留率为(27.6±4.5)%,假手术组分别为(16.0±4.0)min 和(18.1±3.3)%,两组差异有统计学意义(均为 P<0.01)。AMI 组心肌梗死面积为(52.1±4.1)%,与50min 胃内核素残留率呈正相关(r=0.620,P=0.031)。AMI 组、假手术组血浆胃动素分别为(81.5±17.6)ng/L、(103.8±24.9)ng/L,AMI 组较假手术组降低(P<0.01)。结论 AMI 大鼠胃排空明显减慢,胃内核素残留率与梗死而积呈正相关性;胃排空减慢与胃动素分泌减少有关。
Objective To observe the changes of gastric emptying in acute myocardial infarction (AMI) rats and to explore its possible mechanism. Methods Twelve AMI rats (AMI group) and nine sham rats (sham operation group) were treated with 99mTc tin (99m) TcDTPA) Gastric emptying was performed under single photon emission computerized tomography. After AMI, AMI rats were sacrificed and myocardial infarct size was calculated by the staining of 2,3,5-triphenyltetrazolium chloride: AMI group , Sham-operation group rats (n = 9), and radioimmunoassay for determination of plasma motilin. Results The gastric half emptying time (GET_ (1/2)) was (23.1 ± 4.7) min in AMI group, the residual rate of gastric radionuclide in 50 min was (27.6 ± 4.5)% in AMI group and (16.0 ± 4.0) min in sham operation group (18.1 ± 3.3)%, the difference between the two groups was statistically significant (both P <0.01). AMI myocardial infarction area was (52.1 ± 4.1)%, and 50min gastric residual rate was positively correlated (r = 0.620, P = 0.031). The levels of plasma motilin in AMI group and sham operation group were (81.5 ± 17.6) ng / L and (103.8 ± 24.9) ng / L, respectively, lower than those in sham operation group (P <0.01). Conclusion The gastric emptying in AMI rats was significantly slowed down. The residual rate of gastric radionuclides was positively correlated with infarct volume. The slowed gastric emptying was associated with decreased motilin secretion.