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建立了在线渗析-双柱串联离子色谱(IC)法直接检测婴幼儿乳粉中肌醇的方法。样品经蛋白沉淀后,通过在线渗析,采用Metrosep Carb 1(150×4.0 mm,5μm)和M etrosep A SUPP 5(250×4.6 mm,5μm)两根不同分离性质的色谱柱进行分离,流动相为10 mmol/L Na OH溶液,等度洗脱,流速为0.5 m L/min,安培检测器(金电极)直接测定婴幼儿乳粉中的肌醇。方法的线性范围为1.0~100.0 mg/kg,方法检出限为0.5 mg/kg,加标回收率为92.1%~103.8%;相对标准偏差RSD为2.1%~3.5%。
An in-line dialysis-double column tandem ion chromatography (IC) method was established for the direct detection of inositol in infant milk powder. After protein precipitation, the samples were separated by on-line dialysis using two columns with different separation properties: Metrosep Carb 1 (150 × 4.0 mm, 5 μm) and Metrosep A SUPP 5 (250 × 4.6 mm, 5 μm). The mobile phase was 10 mmol / L NaOH solution, isocratic elution, flow rate of 0.5 m L / min, amperometric detector (gold electrode) direct determination of infant inositol in milk powder. The linear range of the method was 1.0-100.0 mg / kg. The detection limit was 0.5 mg / kg. The recoveries were 92.1% -103.8%. The relative standard deviations (RSDs) ranged from 2.1% to 3.5%.