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目的建立以高效液相色谱法同时测定蒙药草乌叶中新乌头碱、乌头碱、次乌头碱的方法。方法Extend-C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.3mol/L三乙胺(65∶35),体积流量为0.8mL/min,检测波长为235nm,柱温为35℃。结果经过方法学考察,本测定方法具有一定的专属性、准确性、重现性和可行性,新乌头碱、乌头碱、次乌头碱分别在1～5、0.35～1.75、0.5～2.5μg呈良好的线性关系,平均加样回收率分别为98.43%、98.98%、98.82%,RSD分别为1.38%、0.89%、1.03%。结论本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。
Objective To establish a method for the simultaneous determination of mesaconitine, aconitine and subaccharine in Mongolian herb leaves by high performance liquid chromatography. Method Extend-C18 column (250mm×4.6mm, 5μm), mobile phase was methanol-0.3mol/L triethylamine (65:35), volume flow rate was 0.8mL/min, detection wavelength was 235nm, column temperature was 35°C . Results After the methodological investigation, the determination method has a certain degree of specificity, accuracy, reproducibility and feasibility. The new aconitine, aconitine, hypaconitine were 1 ~ 5, 0.35 ~ 1.75, 0.5 ~ 2.5μg showed a good linear relationship, the average sample recovery was 98.43%, 98.98%, 98.82%, RSD were 1.38%, 0.89%, 1.03%. Conclusion This method is simple, accurate, good separation effect, wide linear range, high sensitivity, can be used for the quality control of this product.