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用不同手术方式制作单纯胃食管反流 (G组 )、单纯十二指肠食管反流 (D组 )、十二指肠胃混合食管反流 (DG组 )及无反流对照组 (C组 )动物模型 ,于术后不同时期取出食管 ,用免疫组化法检测p16、PCNA、CyclinD1、CDK4 等基因的表达。结果显示 :D组、DG组大鼠食管组织中PCNA、CyclinD1、CDK4 基因表达显著高于G组 ,并随反流时间延长而明显增强 ,反流组与C组相比均有显著差异 ;各组不同时期p16表达相似。免疫组化结果与组织学改变高度一致。说明胃及十二指肠反流可能通过改变细胞周期调控基因的表达而致食管上皮增生 ,促进反流性食管炎及其并发症的发生 ,其中十二指肠内容物的作用更强。
Gastroesophageal reflux (G group), simple duodenal esophageal reflux (D group), duodeno-gastric mixed esophageal reflux (DG group) and no-reflux control group (C group) Animal model, esophagus were taken out at different times after operation, and the expression of p16, PCNA, CyclinD1, CDK4 and other genes were detected by immunohistochemistry. The results showed that the expression of PCNA, CyclinD1 and CDK4 in esophageal tissues of D group and DG group were significantly higher than that of G group and significantly increased with the prolongation of reflux time, and there was significant difference between C group and reflux group The expression of p16 in different groups was similar. Immunohistochemical results and histological changes are highly consistent. Gastric and duodenal regurgitation may be caused by changes in cell cycle regulation of gene expression and esophageal epithelial hyperplasia, and promote the occurrence of reflux esophagitis and its complications, including duodenal contents stronger.