论文部分内容阅读
目的:比较四神丸和葛根芩连片对硫酸葡聚糖钠(DSS)诱导的小鼠急性和慢性结肠炎的治疗作用。方法:急性或慢性结肠炎实验中C57BL/6J小鼠均随机分为4组,即正常组,DSS急性或慢性模型组,四神丸组(2.25 g·kg-1),葛根芩连片组(6.5 g·kg-1)。4%DSS连续给小鼠自由饮用5 d制备急性结肠炎模型;3%DSS给小鼠4次循环饮用(1~5 d,8~12 d,15~19 d,22~26 d)制备慢性结肠炎模型。急性模型于DSS饮水次日开始给药,连续ig给药8 d;慢性模型于DSS饮水2次循环后开始给药,连续给药16 d。分别测定小鼠疾病活动度指数(DAI)和结肠组织中髓过氧化物酶(MPO)活性,HE染色观察结肠组织学变化。结果:与正常组比较,模型组急性结肠炎与慢性结肠炎小鼠DAI均明显升高,结肠组织炎症损伤较为明显,MPO活性明显升高(P<0.05,P<0.01);急性结肠炎中葛根芩连片从第5天开始、四神丸从第7天开始显著改善小鼠DAI(P<0.05),二者均能显著减轻小鼠结肠组织炎症损伤(P<0.01,P<0.05),并降低结肠组织中MPO活性(P<0.05,P<0.01);慢性结肠炎中四神丸从第18至22天及24天可显著改善模型小鼠的DAI(P<0.05),并能减轻结肠组织炎症损伤(P<0.05),葛根芩连片对慢性结肠炎小鼠各指标均无显著性改善。结论:四神丸对DSS诱导的急性及慢性结肠炎均有显著改善作用,葛根芩连片仅对急性结肠炎有显著疗效。
Objective: To compare the therapeutic effects of Si Shen Pill and Gegenqinqian Tablets on acute and chronic colitis induced by dextran sulfate sodium (DSS) in mice. METHODS: C57BL / 6J mice were randomly divided into 4 groups (normal control group, acute or chronic DSS model group), DSS acute or chronic model group (2.25 g · kg -1), Gegen Qinlian tablet group (6.5 g · kg -1). 4% DSS was given to mice for 5 days to make a model of acute colitis. The rats were dredged with 3% DSS for 4 times (1 ~ 5 d, 8 ~ 12 d, 15 ~ 19 d, 22 ~ 26 d) Colitis model. The acute model was started on the next day after DSS drinking water, and the rats were dosed ig for 8 days. Chronic model was started after DSS drinking water twice and dosed continuously for 16 days. The disease activity index (DAI) and the activity of myeloperoxidase (MPO) in the colon were measured respectively. The histological changes of the colon were observed by HE staining. Results: Compared with the normal group, the DAI of acute colitis and chronic colitis mice were significantly increased in the model group, the inflammatory injury of colonic tissue was more obvious and the activity of MPO was significantly increased (P <0.05, P <0.01); in acute colitis Gegen Qin Conjugate began on the 5th day and Sishen Pill significantly ameliorated the DAI of mice from the 7th day (P <0.05), both of which could significantly reduce the inflammatory injury of the colonic tissue in mice (P <0.01, P <0.05) , And decreased MPO activity in colon tissue (P <0.05, P <0.01). In chronic colitis, Sishen Pill significantly improved DAI (P <0.05) in model mice from day 18 to 22 and 24 (P <0.05). Gegen Qinlian contiguous tablet had no significant improvement on all indexes of chronic colitis mice. Conclusion: Sishen pill can significantly improve DSS-induced acute and chronic colitis. Gegen Qinlian contiguous tablet has a significant effect on acute colitis.