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锌指蛋白在原核生物与真核生物基因转录的调控中发挥着重要作用.根据火龙果抗逆cDNA芯片中的一个EST设计一对引物,采用RT-PCR克隆火龙果类锌指蛋白基因片段,生物信息学分析表明,该片段长334bp,编码1中含有109个氨基酸残基的肽链,命名为HuZF.该基因序列与其它植物锌指蛋白基因核苷酸序列的同源性均在70%以上,推测的氨基酸序列与其它植物锌指蛋白的氨基酸序列的同源性达60%以上.RT-PCR分析表明,高盐和干旱胁迫下,火龙果茎中HuZF的表达增强,低温胁迫时,HuZF的表达先减弱后增强,初步推测该基因的表达与火龙果低温、高盐、干旱等逆境胁迫响应相关.
Zinc finger protein plays an important role in the regulation of gene transcription in prokaryotes and eukaryotes.A pair of primers was designed according to one EST in dragon fruit anti-retrograde cDNA microarray, and the zinc finger protein gene fragment of dragon fruit was cloned by RT-PCR, Bioinformatics analysis showed that the fragment was 334 bp in length and encoded 109 amino acid residues in the coding sequence of HuZF, which shared 70% homology with that of other plant zinc finger proteins. The deduced amino acid sequence was more than 60% homologous to the amino acid sequences of zinc finger proteins from other plants.RT-PCR analysis showed that under high salt and drought stress, the expression of HuZF in the stems of Pyracantha fortunei increased. Under low temperature stress, The expression of HuZF first weakened and then increased. It was preliminarily presumed that the expression of HuZF was related to the response to the stress of low temperature, high salt and drought of dragon fruit.