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要了解蛋白质那样复杂的生物高分子,很重要的一种方法是,利用“质量分析”将样品的分子离子化,来了解化合物的重量。20世纪80年代,世界各地都在利用激光进行有机化合物的离子化研究。小分子用这个方法效果不错,可是一遇到大分子,麻烦就出来了。激光被待分析的化合物吸收,其内部的能量就很高,温度过高就会把分子链切断,使分子七零八散,所以当时能够用激光进行离子化的只是那些极少数分子量很小的化合物,其重量范围大概不超过氢重量的1000倍。因此,从当时化学家的常识来看,分子量超过10000,
A very important way to understand a complex biological polymer like protein is to use “mass spectrometry” to ionize the sample molecules to understand the weight of the compound. In the 1980s, lasers were used to ionize organic compounds around the world. Small molecules with this method works well, but once encountered a large molecule, the trouble came out. The laser is absorbed by the compound to be analyzed, its internal energy is very high, the temperature is too high will cut off the molecular chain, the molecules scattered, so was able to use laser ionization only those very few very small molecular weight The weight of the compound should not exceed 1000 times the weight of hydrogen. Therefore, from the common knowledge of chemists at the time point of view, the molecular weight of more than 10,000,