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我们79年进行了杂交水稻幼穗组织培养的实验,现已诱导出愈伤组织并成功分化出完整植株。材料和方法实验材料为黔东南州农科所提供的七个籼型杂交水稻F_1代。当幼穗处于雌雄蕊形成期(长约1厘米左右)从田间植株剥出幼穗,经0.1％升汞消毒10分钟后,用无菌水冲洗2～3次,在无菌条件下,将材料剪成2～3毫米,分别接种在培养基表面。
We experimented on young panicle tissue culture in 79 years and now we have induced callus and successfully differentiated whole plants. Materials and Methods The experiment materials were F_1 generation of seven Indica hybrid rice provided by Agronomy Department of Qiandongnan Prefecture. When the young spike in the period of formation of the pistils (about 1 cm long) from the field plant stripped of young spikes, sterilized by 0.1% mercuric chloride for 10 minutes, rinsed with sterile water 2 to 3 times, under sterile conditions, will Materials cut into 2 to 3 mm, were inoculated on the surface of the medium.