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为了解森林脑炎病毒的增殖动态 ,改进现有疫苗的生产工艺 ,将森林脑炎病毒感染地鼠肾细胞 ,控制不同的培养条件 ,以利病毒增殖 ,并于不同时间收获病毒液 ,测定病毒滴度。疫苗液用不同浓度的福尔马林灭活后 ,测定疫苗的保护指数。结果表明森张株病毒增殖高峰在 6 8～ 72h ,多次收获的病毒液及其病毒滴度、疫苗效力均优于现有疫苗 ,新工艺可提高病毒产量 ,为疫苗的精制纯化奠定了基础
In order to understand the dynamic of proliferation of forest encephalitis virus and improve the production process of the existing vaccines, the virus was infected into the rat kidney cells by encephalitis virus and different culture conditions were controlled to facilitate the proliferation of the virus and the virus solution was harvested at different times, and the virus Titers. After the vaccine solution was inactivated with different concentrations of formalin, the vaccine protection index was determined. The results showed that the peak of proliferation of Morus albopictus was between 68 and 72 hours. The virus fluid and its virus titer and vaccine potency that were harvested several times were better than those of the existing vaccine. The new technology could increase the virus yield and lay a foundation for the purification and purification of the vaccine