Shepard指出用少量活的麻风杆菌接种于许多鼠株的足垫后产生麻风杆菌有规律的局限性繁殖。新近研究证明,在接种大量麻风杆菌24小时后只能回收接种菌的30％。为阐明所接种的细菌的去向,作者用新收获的或在4℃贮存了数周的麻风杆菌、海鱼分枝杆菌和放射性硫化锝~(99)胶体颗粒作了一系列回收试验。 Shepard pointed out that regular inoculation of M. leprae occurred with limited inoculation of many live M. leprae on foot pads in many rat strains. Recent studies have shown that only 30% of inoculated bacteria can be recovered 24 hours after inoculation of large numbers of leprosy bacteria. To illustrate the whereabouts of the inoculated bacteria, the authors conducted a series of recovery tests with newly harvested, or leprosy, Mycobacterium maritima, and radioactive-technetium (99) 99 colloidal particles newly harvested or stored at 4 ° C for several weeks.