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利用SRAP标记技术对来自江苏的51份、浙江的9份和福建的6份共66份杨梅种质进行遗传多样性分析。从64对SRAP引物组合中筛选出57对多态性较好、扩增清晰的引物组合。这57对引物组合共扩增出431条带,其中213条具有多态性,多态率49.4%。66份杨梅种质间的Dice遗传相似系数在0.59~0.85之间。UPGMA聚类分析结果表明,在相似系数0.618处可将66份种质划分为3大类,分别代表福建杨梅、浙江杨梅和江苏杨梅,地理来源相近的品种相对聚合,少数浙江和福建品种与江苏品种间出现交叉聚类。这揭示了杨梅品种间亲缘关系与地理分布以及来源密切相关。
SRAP markers were used to analyze the genetic diversity of Myrica rubra in 51 accessions from Jiangsu, 9 from Zhejiang and 66 from Fujian. From 64 pairs of SRAP primer combinations, 57 pairs of primer combinations with good polymorphism and clear amplification were selected. The 57 pairs of primer combinations amplified a total of 431 bands, of which 213 were polymorphic and the polymorphism rate was 49.4%. The Dice genetic similarity coefficients of 66 accessions were between 0.59 and 0.85. UPGMA clustering analysis showed that 66 germplasms could be divided into three groups at the similarity coefficient of 0.618, which represented the relative polymerizations of the similar geographical origins in Yangmei of Fujian, Yangmei of Zhejiang and Yangmei of Jiangsu, respectively. A few Zhejiang and Fujian cultivars were clustered with Jiangsu Cross-cluster between breeds. This revealed that the genetic relationship between bayberry varieties is closely related to geographical distribution and origin.