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目的 慢性营养不良恢复期可能会发生青春期发动提前 ,本研究探讨恢复期营养干预对内毒素血症致致营养不良幼年雌性大鼠青春发动及IGF 1轴的影响。方法 体重匹配的同龄同窝幼年雌鼠 ,随机分至对照组 (C组 )及LPS处理组 (L组 ) (均n =8)。实验 1,L1组于生后 7- 13d隔日注射内毒素脂多糖 (LPS)共 4次 ,模拟反复感染 ;实验 2 ,L2组于生后 14～ 2 0d同样给予LPS。对照组与相应L组同时注射 0 .1mlPBS ,并予正常饮食。恢复期L1及L2组予高蛋白高热卡饲料喂养至体重赶上相应的C1及C2组。观察雌鼠的阴道口开放 (VO)、第 1个动情 (E)日龄及动情后期 (M期 )卵巢黄体的形成 ;血浆TNFα、IGF 1及IGFBP - 3水平的变化。结果 ①L1组VO及E日龄均与C1组相似 (33.13± 2 .90dvs 33.5 0± 2 .6 7d ;37.13± 2 .5 9dvs 35 .88± 1.81d ,P >0 .0 5 ) ;两组M期卵巢黄体数无统计学差异 (3.75± 1.6 7vs 3.13± 1.13,P >0 .0 5 )。②L1组内毒素血症期末血TNF α水平高于C1组 (1.13± 0 .5 7U/mlvs 0 .71± 0 .18U/ml,P <0 .0 5 ) ;而血IGF 1水平则低于C1组 (2 0 1.6 3±2 8.31ng/mlvs 2 5 0 .91± 35 .0 7ng/ml,P <0 .0 5 )。恢复早期L1组血IGF 1水平回升并高于C1组 (2 89.2 0±39.0 7ng/mlvs 2 4 9.88± 33.5 0ng/ml,P <0 .0
Objective Chronic malnutrition recovery period may occur early onset of puberty. This study was to investigate the effect of convalescent nutrition intervention on endurance-induced juvenile adolescent malnutrition and IGF1 axis in rats. Methods Weight-matched female littermates of the same age were randomly divided into control group (C group) and LPS-treated group (L group) (n = 8). In experiment 1, LPS group was injected with lipopolysaccharide (LPS) for 4 times on the next day after birth to simulate repeated infection. In experiment 2, LPS was also given to LPS at 14-20 days after birth in group L2. The control group and the corresponding L group simultaneously injected 0.1 ml PBS, and to normal diet. Convalescent groups L1 and L2 were fed high-protein high-calorie diet until body weight reached the corresponding C1 and C2 groups. The changes of vaginal opening (VO), formation of corpus luteum on the first (E) day of estrus and late estrus (M), and the changes of plasma TNFα, IGF 1 and IGFBP - 3 were observed. Results ① The VO and E days of L1 group were similar to those of C1 group (33.13 ± 2.90d vs 33.5 ± 2.67d, 37.13 ± 2.59dvs, 35.88 ± 1.81d, P> 0.05) There was no significant difference in ovarian corpus lumens between M stage (3.75 ± 1.6 7 vs 3.13 ± 1.13, P> 0.05). ② The level of TNFα in the endotoxemia group was higher than that in the C1 group (1.13 ± 0.57U / ml vs0.71 ± 0.18U / ml, P <0.05), while the serum IGF1 level was lower than C1 group (20 1.6 3 ± 2 8.31 ng / ml vs 250.091 ± 35.0 7 ng / ml, P <0.05). In the early stage of recovery, the level of IGF1 in serum of group L1 was higher than that of group C1 (2 89.2 ± 39.0 7ng / ml vs 24.898 ± 33.5 0ng / ml, P <0. 0