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目的探讨犬心房纤颤(atrial fibrillation,AF)模型中微囊蛋白-3(Caveolin-3)蛋白表达情况。方法建立犬房颤(AF)模型,通过免疫荧光染色和Western blot技术检测心房快速起搏建立的犬AF模型及AF患者心房肌的Caveolin-3蛋白表达情况。结果在犬和人房颤的心房组织,Western blot结果显示Caveolin-3蛋白表达明显降低,与非房颤组织相比P<0.05。在犬假手术组和窦性心律的患者,免疫荧光结果显示位于心房组织细胞膜上Caveolin-3蛋白表达明显,并均匀分布在细胞膜表面;而在AF状态下,人和犬心房肌Caveolin-3蛋白表达显著降低。结论Caveolin-3参与了AF这一临床复杂病症的发生和发展。
Objective To investigate the protein expression of Caveolin-3 in atrial fibrillation (AF) model of dogs. Methods Atrial fibrillation (AF) model was established. The canine AF model established by rapid atrial pacing and the expression of Caveolin-3 protein in AF patients were detected by immunofluorescence staining and Western blot. Results The results of Western blot showed that the expression of Caveolin-3 protein was significantly decreased in atrial tissues of canine and human atrial fibrillation (P <0.05). In canine sham operation group and sinus rhythm patients, immunofluorescence results showed that the expression of Caveolin-3 protein in atrial tissue membrane was obvious and evenly distributed on the cell membrane surface; while in the AF state, human and canine atrial muscle Caveolin-3 protein Expression was significantly reduced. Conclusion Caveolin-3 participates in the occurrence and development of AF, a complex clinical condition.