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目的 探讨诱导型一氧化氮合酶 (induciblenitricoxidesynthase ,iNOS)与犬良性前列腺增生 (benignprostatichyperplasia ,BPH)病理变化的关系。 方法 建立犬前列腺增生动物模型 ,用免疫组化法研究模型犬各时期iNOS在前列腺组织中的表达。结果 犬前列腺组织中iNOS阳性染色主要分布于腺泡上皮细胞及上皮下组织中 ,间质和平滑肌组织中均为阴性。iNOS的表达随雄激素注射时间延长而降低 ,各时段差异均有显著性 (P <0 .0 5 )。结论 雄激素对前列腺组织内的iNOS起负性调节作用 ,iNOS的减少参与了前列腺增生的病理生理过程
Objective To investigate the relationship between inducible nitric oxide synthase (iNOS) and pathological changes of benign prostatic hyperplasia (BPH) in dogs. Methods The model of canine prostatic hyperplasia was established. The expression of iNOS in prostatic tissue was examined by immunohistochemistry. Results The positive expression of iNOS in canine prostatic tissue was mainly distributed in acinar epithelial cells and subepithelial tissues, and negative in both interstitial and smooth muscle tissues. The expression of iNOS decreased with the prolongation of androgen injection time, and the difference was significant at all time points (P <0.05). Conclusions Androgen plays a negative regulatory role on iNOS in prostate tissue. The decrease of iNOS is involved in the pathophysiological process of benign prostatic hyperplasia