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报道一种人血清蛋白（Humanserumalbumin，HSA）键合固定相的合成方法及DL（±）色氨酸等在HSA柱上的手性分离结果。硅胶与3-aminopropyltrimethoxysilane反应生成氨丙基硅胶，分散于乙腈中，用N，N’-disuccinimidylcarbonate活化。HSA与活化硅胶在20mmol／L磷酸钠缓冲液（pH6．6）中，于30℃反应20h。用RP-HPLC法监控固定相的合成过程。当硅胶与HSA重量比为10：1时，HSA的表面覆盖率为2.75μmol／g。用1％异丙醇的20mmol／LK2HPO4-KH2PO4。缓冲液（pH7．50）作流动相，DL（±）色氨酸可以得到较好的分离，D（＋）异构体先流出色谱柱，k为675，a为1．44，Rs为1．52。DL（±）苯丙氨酸、酪氨酸和组氨酸在上述条件下未得到分离。结果表明：HSA柱对结构相似的氨基酸显示出不同的分离能力。
A synthetic method of human serum albumin (HSA) bonded stationary phase and chiral separation of DL (±) tryptophan on HSA column were reported. Silica gel reacts with 3-aminopropyltrimethoxysilane to give aminopropyl silica dispersed in acetonitrile and activated with N, N’-disuccinimidylcarbonate. HSA and activated silica gel in 20mmol / L sodium phosphate buffer (pH6.6), at 30 ℃ for 20h. The RP-HPLC method was used to monitor the synthesis of the stationary phase. The surface coverage of HSA was 2.75 μmol / g when the weight ratio of silica gel to HSA was 10: 1. 20 mmol / L K2HPO4-KH2PO4 using 1% isopropanol. (±) tryptophan, D (+) isomers elute out of the column, k is 675, a is 1.44 and Rs is 1 .52. DL (±) phenylalanine, tyrosine and histidine were not separated under the above conditions. The results showed that HSA column showed different separation ability for similar amino acids.