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目的：调查本市９８初春“流感”非细菌性病原体的感染状况，为临床诊治提供参考资料。方法：利用ＰＣＲ和核酸序列分析技术，对流感儿童（１０８例）和成人（３８例），进行咽拭子病原微生物的核酸片段扩增及序列分析。结果：（１）本次流感儿童查到Ｂ１９细小病毒（Ｂ１９－Ｖ）、生殖支原体（Ｍｇ）、沙眼衣原体（ＣＴ）各为６／１０８，肺炎支原体（Ｍｐｎ）１８／１０８，肺炎衣原体（ＣＰｎ）６０／１０８，未查到腺病毒（ＡＤ）。本次流感成人查到ＣＴ２／３８，ＭＰｎ８／３８，ＣＰｎ２０／４０，未查到Ｂ１９－Ｖ、Ｍｇ和ＡＤ。所查６种病原体检出率，经ｘ２分析表明，儿童和成人均为ＣＰｎ增高，Ｐ＜０．００１。与非流行时正常儿童（１２０例）比较，ＣＰｎ感染率显著增高，Ｐ＜０．０００１。（２）经核酸序列分析证明，本市ＣＰｎ与国际标准株达到１００％同源性。结论：此次流感感染率ＣＰｎ超过５０％，ＭＰｎ１６．７－２１．１％，说明衣原体及支原体感染，为此次本地区流感特征，应当引起高度重视。
Objective: To investigate the status of infection of “flu” non-bacterial pathogens in the early spring of 98 in our city and provide references for clinical diagnosis and treatment. Methods: PCR and nucleic acid sequence analysis were used to amplify and sequence the nucleic acid fragments of the throat swabs in both children (108 cases) and adults (38 cases). Results: (1) B19 parvovirus (B19-V), Mycoplasma genitalium (Mg), Chlamydia trachomatis (CT) were all 6/108, Mpn 18/108, CPn ) 60/108, no adenovirus (AD) detected. The influenza adults found CT2 / 38, MPn8 / 38, CPn20 / 40, did not find B19-V, Mg and AD. The detection rate of the six pathogens examined by x2 analysis showed that children and adults were increased CPn, P <0.001. Compared with non-prevalent children (120 cases), CPn infection rate was significantly higher (P <0.0001). (2) Nucleic acid sequence analysis proved that this city CPn and international standard strains achieve 100% homology. Conclusions: The prevalence of influenza infection in this area is over 50% and MPn is between 16.7-21.1%, indicating that Chlamydia and Mycoplasma infection are the characteristics of flu in this area and should be given high priority.