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目的了解上海市黄浦区15家涉禽场所禽流感病毒污染情况,并对消毒效果进行评估。方法采集15家涉禽场所中消毒前后的禽类标本和环境标本,比较消毒前后标本检测的阳性率;利用反转录聚合酶链反应(RT-PCR)进行病毒核酸检测,对消毒前后禽流感病毒亚型的分布进行描述。结果禽类标本消毒前总阳性率为52.86%(37/70),消毒后为47.50%(19/40),差异无统计学意义(P>0.05);环境标本消毒前总阳性率为63.49%(80/126),消毒后为5.26%(4/76),差异有统计学意义(P<0.01)。禽流感病毒亚型分布显示,禽类标本消毒前阳性株的构成以H9、H7N9及甲型未分型为主,消毒后以H5、H7N9及H9为主;环境标本消毒前阳性株的构成以H5、H9及甲型未分型为主,消毒后仅有H9和H5亚型检出。结论消毒措施对涉禽场所环境标本有一定效果。
Objective To understand the contamination of avian influenza viruses in 15 wading places in Huangpu District, Shanghai and to evaluate the disinfection effect. Methods The samples of avian and environmental samples before and after disinfection were collected from 15 Wicked places, and the positive rate of samples before and after disinfection was compared. The detection of virus nucleic acid before and after disinfection by using reverse transcription-polymerase chain reaction (RT-PCR) Type of distribution to describe. Results The total positive rate of the samples before disinfection was 52.86% (37/70) and that after disinfection was 47.50% (19/40), the difference was not statistically significant (P> 0.05). The total positive rate of environmental samples before disinfection was 63.49% 80/126), and 5.26% (4/76) after disinfection, the difference was statistically significant (P <0.01). The distribution of avian influenza virus subtypes showed that the positive strains of pre-disinfection birds were mainly H5, H7N9 and non-subtype H5N1. H5, H7N9 and H9 were the main components of the positive samples after disinfection. The positive H5, , H9 and type A were not subtype, only H9 and H5 subtype were detected after disinfection. Conclusion The disinfection measures have some effects on the environment specimens of Waders.