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目的:探讨系统性红斑狼疮(SLE)血清抗核小体抗体(AnuA)浓度与脱氧核糖核酸酶Ⅰ(DNaseⅠ)活性的关系。方法:采用DNA-甲基绿比色法和间接ELISA法检测76例SLE患者(活动期40例,缓解期36例)与50例健康对照组DNaseⅠ的活性和血清AnuA浓度,并分析二者之间关系。结果:SLE患者血清DNaseⅠ活性水平明显低于健康对照组(P<0.01),而疾病活动期与缓解期相比未见明显差别(P>0.05);与健康对照组相比,血清AnuA浓度升高明显(P<0.01),并且疾病活动期浓度高于缓解期(P<0.01);AnuA浓度和DNaseⅠ的活性成负相关关系(r=-0.43,P<0.01)。结论:SLE患者中DNaseⅠ的活性下降可能导致血清AnuA的浓度升高。
Objective: To investigate the relationship between serum anti-nucleosome antibody (AnuA) concentration and DNase Ⅰ activity in patients with systemic lupus erythematosus (SLE). Methods: DNase Ⅰ activity and serum AnuA concentration were measured in 76 patients with SLE (active stage of 40 cases, remission stage of 36 cases) and 50 healthy controls by DNA-methyl green colorimetry and indirect ELISA. Relationship between. Results: Serum DNase I activity in patients with SLE was significantly lower than that in healthy controls (P <0.01), but there was no significant difference between active and remission stages of SLE patients (P> 0.05). Compared with healthy controls, the serum AnuA concentration (P <0.01). The concentration of AnuA and DNase Ⅰ activity was negatively correlated (r = -0.43, P <0.01). CONCLUSIONS: Decreased DNase I activity in SLE patients may result in an elevated serum AnuA level.