本文利用硅酸乙酯 (TEOS)的溶胶凝胶过程对葡萄糖氧化酶 (GOD)进行了固定 ,并制备了不同载体下的 GOD酶柱。实验结果表明 ,GOD可在 Si O2 的溶胶凝胶中保持较高的活性。将此酶柱用于流动注射化学发光分析体系 ,可实现对葡萄糖的在线检测 ,测定的线性范围为 10～ 10 0 0 μm ol/ L,检出限为 0 .8μmol/ L,RSD为 1.6 %。此传感器的使用寿命长 ,测定结果与新国标方法——酶 -比色法的测定结果相吻合 In this paper, glucose oxidase (GOD) was immobilized by sol-gel process of TEOS, and GOD enzyme columns were prepared under different carriers. The experimental results show that GOD can maintain high activity in the sol of Si O2. The enzyme column was used for the flow-injection chemiluminescence analysis system, which could realize the on-line detection of glucose. The linear range was 10 ~ 100 μmol / L, the detection limit was 0.8 μmol / L and the RSD was 1.6% . The sensor’s long life, the determination of results with the new national standard method - enzyme - colorimetric assay results consistent