Objective:To analyze expression heterogeneity of Integrin beta 3(ITCB3) and B-cell lymphoma2(BCL-2) in lung adenocarcinoma tissue and adenocarcinoma cell line and further provide theoretical direction for molecular biological research of lung adenocarcinoma.Methods:Tissue microarray was used to observe relation among expression,heterogeneitpy and clinical characteristics of ITGB3 and BCL-2 in lung cancer.Results:ITGB3 and BCL-2 increased significantly in A549 cells in CAFs group with β-actin as control:the expression level of BCL-2also increased in 1TGB3 transfected cells with CFP plasmid transfected A549 cells as control:immunohistochemistry staining showed that positive ratcs of 1TGB3,ITGB1 and BCL-2 in normal lung tissues were 0.the positive rates in lung adenocarcinoma were 7.049%,84.51%and 4.23%,respectively:in the results of immunohistochemistrv staining,the expression of Girdin protein in lung adenocarcinoma was homogeneous,however protein expression of 1TCB3,ITGB1 and BCL—2showed different patterns in the same location with significant heterogeneity;majority of ITGB3,TTCB1 or BCL—2 positive tissue showed heterogeneity that expression in trailing edge was higher than that of trailing edge in lung adenocarcinoma tissue,the patients with BCL-2 heterogeneity showed higher lymph node metastasis ratio and lower clinical stage(P<0.05);and the expression of ITGB3 and the clinical characteristics of patients were not significant related(P>0.05).Conclusions:Expression of ITGB3 and BCL-2 in lung adenocarcinoma and adenocarcinoma cell line showed heterogeneity that expression in trailing edge was higher than that of trailing edge,which may play an important role in promoting tumor lymph node metastasis and vascular invasion,and provides a new research direction for exploration of lung adenocarcinoma metastasis mechanism. Objective: To analyze the heterogeneity of Integrin beta 3 (ITCB3) and B-cell lymphoma2 (BCL-2) in lung adenocarcinoma tissue and adenocarcinoma cell line and further provide theoretical direction for molecular biological research of lung adenocarcinoma. Methods: Tissue microarray was used to observe relation among expression, heterogeneitpy and clinical characteristics of ITGB3 and BCL-2 in lung cancer. Results: ITGB3 and BCL-2 increased significantly in A549 cells in CAFs group with β-actin as control: the expression level of BCL-2 increased in 1TGB3 transfected cells with CFP plasmid transfected A549 cells as control: immunohistochemistry staining showed that positive ratcs of 1TGB3, ITGB1 and BCL-2 in normal lung tissues were 0. The positive rates in lung adenocarcinoma were 7.049%, 84.51% and 4.23% respectively: in the results of immunohistochemistrv staining, the expression of Girdin protein in lung adenocarcinoma was homogeneous, but protein expression of 1TCB3, ITGB1 and BCL-2s of the same locations with significant heterogeneity; majority of ITGB3, TTCB1 or BCL-2 positive tissue showed heterogeneity that expression in trailing edge was higher than that of trailing edge in lung adenocarcinoma tissue, the patients with BCL-2 heterogeneity involvement higher lymph node metastasis ratio and lower clinical stage (P <0.05); and the expression of ITGB3 and the clinical characteristics of patients were not significantly related (P> 0.05) .Conclusions: Expression of ITGB3 and BCL-2 in lung adenocarcinoma and adenocarcinoma cell line showed heterogeneity that expression in trailing edge was higher than that of trailing edge, which may play an important role in promoting tumor lymph node metastasis and vascular invasion, and provide a new research direction for exploration of lung adenocarcinoma metastasis mechanism.