采用 Ｃ１８ 反相色谱柱法测定定值全血（ Ｂ１０ Ｒ Ａ Ｄ） ，３ 个浓度的水平。结果：均在预期范围内，其最低检出浓度为２５ ｎｇ·ｍｌ－ １ ，至少在５０ ～１ ０００ ｎｇ·ｍｌ － １ 范围内呈线性；绝对回收率为９９６ ％ ～１０８８ ％ ；批内和批间变异系数分别为７２ ％ 和９３ ％ 。 The C18 reversed-phase column was used to determine the level of B10-R A D in three concentrations. Results: All of them were within the expected range. The minimum detectable concentration was 25 ng · ml-1, linear within the range of at least 50-1 000 ng · ml-1. The absolute recoveries ranged from 996% to 1088% Respectively; the coefficient of variation between in-batch and inter-batch were 72% and 93% respectively.