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目的 观察血管紧张素一型受体拮抗剂缬沙坦对大鼠系膜增生性肾炎病理及相关临床指标的干预作用。方法 采用大鼠Thy 1肾炎模型 ,将肾炎大鼠分为Thy 1肾炎组及缬沙坦治疗组。检测两组疾病诱导后第 1、3、5、7天平均动脉血压、2 4h尿蛋白排泄量、血浆肌酐及尿素氮 ,并取肾脏行光镜及电镜检查 ,免疫组化检测肾小球内PCNA蛋白表达。同时设正常对照。结果 同肾炎组比较 ,缬沙坦治疗组第 3～ 7天 2 4h尿蛋白排泄量减少 (P <0 0 5 ) ,肾小球有核细胞增多程度下降 (P <0 0 5 ) ,系膜细胞增生减少 ,系膜区扩张也减少 (P <0 0 5 ) ,肾小球内PCNA阳性核减少 (P <0 0 5 )。平均动脉血压、血浆肌酐及尿素氮较正常对照无显著差异。结论 对大鼠系膜增殖性肾炎 ,缬沙坦能一定程度抑制系膜增生 ,减轻肾小球病理损伤 ,降低蛋白尿
Objective To observe the effect of valsartan, an angiotensin type 1 receptor antagonist, on the pathology and related clinical indexes of rat mesangial proliferative glomerulonephritis. Methods Rat Thy 1 nephritis model was used. Nephritis rats were divided into Thy1 nephritis group and valsartan treatment group. The mean arterial blood pressure, 24 h urinary protein excretion, plasma creatinine and urea nitrogen were measured on the 1st, 3rd, 5th and 7th days after induction of the disease. The kidneys were examined by light microscope and electron microscope. PCNA protein expression. At the same time set the normal control. Results Compared with the nephritis group, the urinary excretion of urinary excretion of the valsartan group decreased (P <0 05) at 2-4 days and the proliferation of glomerular nucleated cells decreased (P 0 05) Cell proliferation decreased, mesangial expansion also decreased (P <0 05), glomerular PCNA positive nucleus decreased (P <0 05). Mean arterial pressure, plasma creatinine and urea nitrogen than the normal control no significant difference. Conclusion In rat mesangial proliferative glomerulonephritis, valsartan can inhibit mesangial proliferation to a certain degree, reduce glomerular pathological injury and reduce proteinuria