论文部分内容阅读
目的了解郴州市外环境中H5、H7、H9亚型禽流感病毒的动态分布情况,为郴州市人禽流感防控提供参考依据。方法收集2011~2014年郴州市禽流感监测采集的禽流感外环境标本,采用荧光RTPCR法检测流感病毒A型核酸,阳性者进一步作H5、H7、H9亚型的核酸分型检测。监测数据采用Excel软件进行统计分析。结果 2011~2014年共采集了941份禽流感外环境标本,流感病毒A型总阳性检出率为28.91%,H5、H9型高致病性禽流感病毒阳性检出率较高,分别为9.99%和9.56%,2013年和2014年均检测出H7亚型。时间分布上,禽流感主要集中在冬春季;不同的采样场所,城乡活禽市场的阳性检出率最高(35.08%);不同采样部位,宰杀或摆放禽肉案板表面拭子(48.72%)和清洗禽类污水(39.23%)的阳性检出率较高。结论郴州市城乡活禽市场和家禽规模养殖场等外环境中常年有H5、H7、H9等多种亚型禽流感病毒存在,且冬春季节禽流感病毒流行较为活跃,有感染人的风险,应积极采取综合性防控措施。
Objective To understand the dynamic distribution of H5, H7 and H9 subtype avian influenza viruses in the outer environment of Chenzhou City and provide a reference for the prevention and control of bird flu in Chenzhou City. Methods The peripheral blood samples of bird flu collected from the monitoring of bird flu in Chenzhou City from 2011 to 2014 were collected. The influenza A virus nucleic acids were detected by fluorescent RTPCR. The positives were further detected by H5, H7 and H9 genotypes. Monitoring data using Excel software for statistical analysis. Results A total of 941 samples of peripheral blood samples were collected from 2011 to 2014. The total positive detection rate of influenza A virus was 28.91%. The positive detection rates of H5 and H9 HPAI were 9.99 % And 9.56%, H7 subtype was detected in 2013 and 2014 respectively. In the time distribution, the bird flu mainly concentrated in the winter and spring. The positive detection rate was highest (35.08%) in the different sample sites and in the urban and rural live poultry market. The surface swabs (48.72%) of slaughtered or placed poultry cases at different sampling sites, And cleaning of bird sewage (39.23%) positive detection rate is higher. Conclusion There are perennial H5, H7, H9 and many other avian influenza viruses in Chenzhou urban and rural live poultry market and poultry scale farms, and the prevalence of avian influenza virus in winter and spring is relatively active, Should take active and comprehensive prevention and control measures.