论文部分内容阅读
目的探讨低剂量西维因和氰戊菊酯联合染毒对雌性大鼠子宫系统和机体氧化应激的影响。方法将40只清洁级成年雌性SD大鼠按2×2析因设计随机分为4组,分别为阴性对照(玉米油)组、西维因单独染毒组(1/50LD50,11.2 mg/kg)、氰戊菊酯单独染毒组(1/50 LD50,9.02 mg/kg)和西维因(11.2 mg/kg)+氰戊菊酯(9.02 mg/kg)联合染毒组,每组10只。采用经口灌胃方式进行染毒,每天1次,每周6 d,连续染毒8周。末次染毒24 h后,测量大鼠体重和子宫重量,并计算子宫系数;测定血清中超氧化物歧化酶(SOD)、谷胱甘肽S转移酶(GST)活力和还原性谷胱甘肽(GSH)、丙二醛(MDA)含量以及子宫一氧化氮合成酶(NOS)活力。结果西维因和氰戊菊酯联合染毒对雌性大鼠体重、血清中GST水平均无交互作用(P>0.05);对大鼠子宫系数、血清中MDA含量均存在拮抗作用(P<0.05);对血清中SOD活力、GSH含量及子宫NOS活力存在协同作用(P<0.05,P<0.01)。结论西维因和氰戊菊酯联合染毒对大鼠子宫系统存在联合毒性作用,可能是通过破坏氧化-抗氧化系统的平衡和影响子宫组织中NOS的活力等导致子宫功能的降低。
Objective To investigate the effects of low dose of Cimetidine combined with fenvalerate on the uterine system and oxidative stress in female rats. Methods 40 adult female Sprague Dawley rats were randomly divided into 4 groups according to 2 × 2 factorial design. They were negative control (corn oil) group, ), Fenvalerate alone (1/50 LD50, 9.02 mg / kg) and cimetidine (11.2 mg / kg) + fenvalerate (9.02 mg / kg) only. The oral administration of gavage was performed once a day for 6 days a week for 8 weeks. After the last 24 hours of exposure, body weight and uterus weight were measured and the uterine coefficient was calculated. The activities of serum superoxide dismutase (SOD), glutathione S transferase (GST) and reducing glutathione GSH), malondialdehyde (MDA) content and uterine nitric oxide synthase (NOS) activity. Results There was no interaction between body weight and serum GST level in female rats (P> 0.05), and the effect on uterine coefficient and serum MDA content was antagonistic (P <0.05 ); There was synergistic effect on serum SOD activity, GSH content and uterine NOS activity (P <0.05, P <0.01). Conclusion Combined administration of Cimetidine and Fenvalerate exerts a combined toxic effect on the uterus of rats, which may result in the reduction of uterine function by destroying the balance of oxidation-antioxidant system and affecting the vitality of NOS in uterine tissue.