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目的观察退热解毒灵水煎剂对甲型H1N1流感病毒的抑制作用。方法体外抗病毒实验:将MDCK细胞悬液以105/ml接种于96孔板,用细胞维持液连续2倍系列稀释退热解毒灵水煎剂成5000、2500、1250、625、312.5、156.25、78、39、19.5mg/L9个稀释度,另将病毒唑注射液稀释成125、62.5、31.3、15.6、7.8、3.9、2.0、1.0、0.5mg/L9个稀释度,观察细胞病变效应,计算病毒抑制率。体内抗病毒实验:将80只BALB/c小鼠随机分为对照组、模型组、达菲组、退热解毒灵组,每组20只。除对照组外,其他各组小鼠均以10倍半数致死量剂量鼻腔接种甲型H1N1流感病毒50μl。感染当天开始给药,退热解毒灵组灌胃给予退热解毒灵水煎剂每天4133g/kg;达菲组灌胃给予达菲胶囊每天20mg/kg。每组取10只连续观察小鼠存活率;另10只于首次给药后4天、8天各处死5只,测定鼠肺湿质量及肺内病毒半数组织培养感染量(TCID50)。结果体外抗病毒实验:退热解毒灵水煎剂浓度为5000、2500、1250、625、312.5mg/L组和病毒唑注射液浓度为125、62.5、31.3、15.6mg/L组病毒抑制率均在50%以上。体内抗病毒实验:模型组小鼠存活率为0,达菲组与退热解毒灵组存活率分别为90%、60%。染毒后第4天、第8天,达菲组、退热解毒灵组肺湿质量及肺内病毒TCID50与模型组比较均明显下降(P<0.05或P<0.01)。结论退热解毒灵水煎剂对体内、体外甲型H1N1流感病毒均有一定的抑制作用。
Objective To observe the inhibitory effect of antipyretic and detoxifying water decoction on influenza A (H1N1) virus. Methods In Vitro Antiviral Experiment: MDCK cell suspension was inoculated in 96-well plates at 105/ml, serially diluted with cell maintenance solution twice as the antipyretic and detoxifying water decoction into 5000, 2500, 1250, 625, 312.5, 156.25, Dilutions of 78, 39, and 19.5 mg/L, and dilution of ribavirin into 125, 62.5, 31.3, 15.6, 7.8, 3.9, 2.0, 1.0, and 0.5 mg/L 9 dilutions, respectively, to observe cytopathic effect, calculation Virus inhibition rate. In vivo antiviral experiments: 80 BALB/c mice were randomly divided into a control group, a model group, a Tamiflu group, and an antipyretic and detoxification group, with 20 mice in each group. Except for the control group, mice in each group were inoculated intranasally with 50 μl of influenza A H1N1 virus at a dose of 10 times the median lethal dose. On the day of infection, the group was given intragastric administration of antipyretic and detoxifying water detoxification agent at a dose of 4133 g/kg daily; the Duffy group was intragastrically administered with Daffy capsules at a dose of 20 mg/kg daily. Ten mice in each group were continuously observed for the survival rate of the mice; the other 10 rats were sacrificed at 4 days and 8 days after the first administration, and the wet lung mass of the mice and half of the virus in tissue culture (TCID50) were measured. Results Antiviral effect in vitro: The inhibitory rates of antipyretic and detoxifying water detoxification agents in the concentrations of 5000, 2500, 1250, 625, 312.5 mg/L and ribavirin injections were 125, 62.5, 31.3, and 15.6 mg/L. More than 50%. In vivo antiviral experiments: The survival rate of mice in the model group was 0, and the survival rates of the Tamiflu group and the antipyretics and Jieduling group were 90% and 60%, respectively. On the 4th and 8th days after exposure, the lung wet weight and the TCID50 in the lungs of the Tamiflu and Jiefeijieduling groups were significantly lower than those in the model group (P<0.05 or P<0.01). Conclusion The antipyretic and detoxifying water decoction has a certain inhibitory effect on the H1N1 influenza virus in vivo and in vitro.