1991年9月至1992年5月在阿根廷布宜诺斯艾利斯的拉帕拉塔屠宰场,随机抽样采集了体重为90—110kg的109头猪的血清和隔肌样本。用IFAT检测血清弓形虫IgG抗体,并用免疫印渍技术测定血清中抗体与虫体抗原的反应性。隔肌经切碎、制匀浆、消化、离心处理后,制成混悬液,然后腹腔注射接种5—7周龄的ICR小鼠。接种后监测28天,在此期间如发现死亡小鼠,即抽取腹腔渗 Serum and septum samples from 109 pigs weighing 90-110 kg were collected randomly from September 1991 to May 1992 at the La Paracta slaughterhouse in Buenos Aires, Argentina. Serum Toxoplasma gondii IgG antibody was detected by IFAT, and the reactivity of the antibody in serum with the parasite antigen was measured by immunoblotting. The diaphragm muscle was shredded, homogenized, digested and centrifuged to prepare a suspension. The mice were then injected intraperitoneally with 5-7 week-old ICR mice. After vaccination monitoring for 28 days, during which dead mice were found, ie peritoneal dialysis was drawn