胆囊癌与雌激素受体(ER)和孕激素受体(PR)的代谢异常有关。本实验采用免疫组织化学SP法检测胆囊良、恶性组织中的ER、PR、细胞增殖核抗原(PCNA)、 c-erbB2及p53表达水平。一、材料与方法 1．标本来源选取我院1992年～2003 年手术切除且病理证实的胆囊癌40例, 慢性胆囊炎11例,胆囊腺瘤9例。 2．免疫组织化学:判断标准:阴性对照以磷酸盐缓冲液代替一抗。阳性对照为福州迈新公司提供的阳性对照切片。切片中阳性细胞率≥20％为阳性,<20％为阴性。 Gallbladder cancer is associated with abnormal metabolism of estrogen receptor (ER) and progesterone receptor (PR). In this study, immunohistochemical SP method was used to detect the expression of ER, PR, PCNA, c-erbB2 and p53 in benign and malignant gallbladder tissues. First, materials and methods 1. Specimens from 1992 to 2003 in our hospital surgical excision and pathological confirmed 40 cases of gallbladder cancer, chronic cholecystitis in 11 cases, 9 cases of gallbladder adenoma. 2. Immunohistochemistry: Criteria: Negative control with phosphate buffer instead of primary antibody. Positive control Fuzhou Mai new company provides positive control section. Positive cells in the section rate of ≥ 20% was positive, <20% was negative.