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检测水中的霍乱弧菌,一般采用1升水样培养法,近年来又发展了抗体制动技术。此处报告的是一种检测污染水中霍乱弧菌Eltor型的新方法,即纱布过滤增菌培养法。基本操作如下:(1)滤器:将容纳1磅量的塑料广口瓶的底部挖一个孔径约为2 cm的孔作为滤器。该滤器用3%H_2O_2浸泡15分钟消毒,再用灭菌蒸馏水冲洗以后,在清洁(但不是无菌)的条件下均匀地塞入12英寸宽的纱布条。要求松紧适合(即压紧后约占空容器体积的三分之二),使水滤过时不致产生沟隙,然后把此种滤器封置于消毒的塑料袋内备用。(2)采样:在现场打开塑料袋,于塑料瓶底部插一漏斗,通过该滤器采水样10升,然后将纱布及吸着的水一
Detection of Vibrio cholerae in water, the general use of 1 liters of water-like culture, in recent years has developed an antibody braking technology. Here is a report of a new method for the detection of Eltor type Vibrio cholerae in polluted water, ie, gauze filter enrichment culture. The basic operation is as follows: (1) Filter: Dig a hole of about 2 cm in diameter on the bottom of a 1-pound plastic jug as a filter. The filter was sterilized by soaking in 3% H 2 O 2 for 15 minutes and rinsed with sterilized distilled water, then evenly packed into 12 inch wide gauze strips under clean (but not sterile) conditions. Requires tight fit (that is, about two-thirds of the volume of the container after pressing), so that no water will be generated when the filter gap, and then the filter is placed in sterile plastic bags spare. (2) Sampling: Open the plastic bag at the scene, insert a funnel at the bottom of the plastic bottle and adopt the filter to collect 10 liters of water, then put the gauze and the sucked water