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目的建立高效液相色谱法测定油茶中黄曲霉素B1、B2、G1、G2的方法。方法样品用甲醇-水溶液提取、经免疫亲和柱净化、由高效液相分离、0.05%碘溶液衍生化后,以荧光检测器检测,外标法定量。结果黄曲霉素B1、B2、G1、G2均在0.10~2.5μg/L的浓度范围内线性良好,相关系数均γ>0.999 7。样品检出限0.05μg/kg;样品加标回收率为79.5%~92.0%,相对标准偏差为2.7%~3.9%。结论本方法简便快捷、灵敏准确,能够满足武陟油茶中黄曲霉素B1、B2、G1、G2含量测定要求。
Objective To establish a method for the determination of aflatoxins B1, B2, G1 and G2 in Camellia oleifera by high performance liquid chromatography. Methods The samples were extracted with methanol-water solution, purified by immunoaffinity column, separated by high performance liquid phase, derivatized with 0.05% iodine solution, detected by fluorescence detector and quantified by external standard method. Results Aflatoxin B1, B2, G1 and G2 all had good linearity in the concentration range of 0.10-2.5 μg / L with the correlation coefficients γ> 0.999 7. The detection limit was 0.05μg / kg. The spiked recoveries were 79.5% ~ 92.0% with relative standard deviations of 2.7% ~ 3.9%. Conclusion The method is simple, sensitive and accurate, and can meet the determination of aflatoxins B1, B2, G1 and G2 in Wuyue Camellia.