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目的探讨植物乳杆菌M1-UVs29在不同条件下降解胆固醇的能力。方法将M1-UVs29干菌粉活化后,分别接种至MRS液体培养基、MRS-胆盐培养基、MRS-胆固醇培养基和MRS-胆盐-胆固醇培养基中培养,绘制M1-UVs29在不同培养基中的生长曲线;以胆固醇降解率为指标,考察M1-UVs29在不同条件下(胆固醇、胆盐添加量及菌种数量)降解胆固醇的能力。结果胆盐具有抑制菌体生长的作用,胆固醇具有缓解胆盐对菌体生长抑制的作用。随着培养基中胆固醇含量的逐渐增加,胆固醇降解率也随之升高,趋势相对稳定,培养基中胆盐含量为0.2%,胆固醇含量为400μg/ml时,胆固醇降解率可达34.76%;随着培养基中胆盐含量的逐渐增加,胆固醇降解率也增加,但不呈规律性变化,培养基中胆固醇含量为100μg/ml,胆盐含量为0.4%时,胆固醇降解率可达32.85%;培养基中胆盐含量大于0.5%,胆固醇降解率极剧下降;培养基中胆固醇含量为100μg/ml,胆盐含量为0.2%,菌种数量为2×108cfu/ml时,胆固醇降解率达最高,为37.30%。结论植物乳杆菌M1-UVs29具有稳定的降解胆固醇的能力,本实验为其进一步应用提供了参考。
Objective To investigate the ability of Lactobacillus plantarum M1-UVs29 to degrade cholesterol under different conditions. Methods After the M1-UVs29 dry bacterial cells were activated, they were inoculated into MRS liquid medium, MRS-bile salt medium, MRS-cholesterol medium and MRS-bile salt-cholesterol medium respectively. Based on the growth curve; cholesterol degradation rate as an indicator to investigate M1-UVs29 under different conditions (cholesterol, bile salt dosage and the number of bacteria) ability to degrade cholesterol. Results Bile salt has the effect of inhibiting cell growth. Cholesterol has the effect of relieving bile salts on the growth inhibition of the cells. With the gradual increase of cholesterol in culture medium, cholesterol degradation rate also increased, the trend is relatively stable, the bile salt content in culture medium is 0.2%, the cholesterol degradation rate is up to 34.76% when the cholesterol content is 400μg / ml; With the gradual increase of the bile salt content in the medium, the cholesterol degradation rate also increased, but not regular changes, the cholesterol content of the medium was 100μg / ml, the bile salt content of 0.4%, cholesterol degradation rate of up to 32.85% ; The bile salt content in the medium was more than 0.5% and the rate of cholesterol degradation dropped dramatically; the cholesterol content in the medium was 100μg / ml, the bile salt content was 0.2% and the number of bacteria was 2 × 108cfu / ml, the cholesterol degradation rate reached Highest, 37.30%. Conclusion Lactobacillus plantarum M1-UVs29 has a stable ability to degrade cholesterol. This experiment provides a reference for its further application.