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p53基因是一种抑癌基因,它在肿瘤发生过程中有重要作用.p53基因的突变可引起细胞增殖,导致悼瘤的发生.迄今已在许多恶性肿瘤细胞中发现p53基因的突变.将外源野生型p53基因导入p53基因突变的肿瘤细胞,能抑制其恶性增殖,逆转其恶性表型.这些研究为肿瘤的基因治疗奠定了基础;值得注意的是,至今大多数研究均用有p53基因缺失或突变的肿瘤细胞,但并非所有的肿瘤细胞都发生p53基因突变;本文将外源野生型p53基因导入表达内源野生型p53基因的膀胱癌细胞株EJ,观察其抑制效应及凋亡的诱导作用.
p53 gene is a tumor suppressor gene that plays an important role in tumorigenesis.The mutation of p53 gene can cause cell proliferation and lead to the occurrence of mummoma.The mutation of p53 gene has been found in many malignant tumor cells so far The introduction of the wild-type p53 gene into the p53-mutated tumor cells can inhibit its malignant proliferation and reverse its malignant phenotype.These studies laid the foundation for the gene therapy of tumors.It is worth noting that most of the studies so far have used the p53 gene Deletion or mutation of tumor cells, but not all tumor cells have p53 gene mutation; In this paper, wild-type p53 gene into wild-type p53 gene expression in bladder cancer cell line EJ, observed its inhibitory effect and apoptosis Induction.