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目的 :介绍一种省时、高效的构建 T-载体的方法。方法 :在完全消化质粒的 3末端加上一个胸腺嘧啶 ( T)。结果 :构建了两种 T-载体 :p GEM- 5Z和 pc DNA3。结论 :这种 T-载体可以非常有效地用于 PCR产物的克隆。
Objective: To introduce a time-saving and efficient method of constructing T-vector. Method: A thymine (T) was added to the 3 end of the completely digested plasmid. Results: Two T-vectors were constructed: p GEM-5Z and pc DNA3. Conclusion: This T-vector can be used very efficiently for the cloning of PCR products.