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1引言采用高效液相色谱（HPLC）法分离单糖和寡糖，较多采用氨基柱，乙附和水作为流动相，此法具有完全分离麦汁、发酵液和啤酒中的果糖、葡萄糖、蔗糖、麦芽糖和麦芽三糖的优点。本文采用hoN比往分析麦汁、发酵液和啤酒中三糖以内的可发酵糖。至今尚未见到采用反相高效液相色
1 Introduction High performance liquid chromatography (HPLC) separation of monosaccharides and oligosaccharides, the more the use of amino, acetonitrile and water as the mobile phase, this method has a complete separation of wort, fermentation broth and beer, fructose, glucose, sucrose Advantages of maltose and maltotriose. In this paper, the ratio of hoN was used to analyze the fermentable sugars in wort, fermentation broth and beer trisaccharide. Up to now have not seen the use of RP-HPLC