OBJECTIVE: To evaluate the efficacy of Renshen Sanqi Chuanxiong formula(RSCF)for preventing vascular aging,and to investigate the possible mo-lecular mechanism underlying the actions of RSCF.METHODS: Potentially active components and their relatively direct targets were identified by combining drug-likeness(DL)screening using a tar-get identification process.Vascular aging-associat-ed targets for RSCF were obtained by selecting common genes not only from potential targets but also from human vascular aging-associated genes.Cytoscape 3.2.1 software was employed to visual-ize the complex compound-target and target-func-tion networks.Biological process and molecular function were assessed,and the Kyoto Encyclope-dia of Genes and Genomes and pathway enrich-ment analyses were performed using ClueGO.Path-ways directly associated with vascular aging were integrated into a vascular aging-related pathway.RESULTS: Altogether,122 potentially active compo-nents of RSCF were identified through DL screen-ing,and their corresponding 692 direct targets were retrieved via target prediction and identifica-tion.We identified 49 vascular aging-associated tar-gets for RSCF by overlapping the 692 potential tar-gets with 146 human vascular aging-associated genes.The results from the compound-target net-work indicated that most components acted on common targets and displayed synergistic action,which showed that the magnifying effects of RSCF were based on these common targets.The tar-get-function network revealed that each target was involved in multiple function modules,suggesting that RSCF was multi-functional during treatment of vascular aging.The results of the ClueGO analysis indicated that most of the targets were associated with the hypoxia-inducible factor 1(HIF-1)signal-ing pathway.The results from the pathway analysis also indicated that an integrative vascular aging-re-lated pathway mainly included an angiogenesis regulation module,cell-survival module,and oxida-tive stress-resistance module.rnCONCLUSION: Our results suggested that many components act synergistically on common targets to delay vascular aging,and each target is involved in multiple functional modules.The ClueGO analy-sis indicated that most of the targets were connect-ed to the HIF-1 signaling pathway,FOXO signaling pathway,and thyroid hormone signaling pathway.