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目的检测大鼠死后不同器官能量物质的改变情况,探讨死后能量物质的变化规律。方法用高效液相色谱检测死亡发生24h内不同时间点大鼠腓肠肌、心肌、肝脏组织中ATP、ADP和AMP的含量。结果发现腓肠肌中ATP随死亡时间的延长迅速下降,到死后8h基本检测不到或微量检测出;心肌、肝脏中组织中ATP含量受死亡方式影响大。ADP在整个检测时间点均能检测到,AMP的含量变化无规律。结论通过高效液相对磷酸腺苷的检测,显示24h内大鼠腓肠肌、心肌和肝脏组织中的ATP含量变化与死亡时间的联系,可为法医学死亡时间推断提供一种新的、客观的参考依据。
Objective To detect the changes of energy substances in different organs after the death of rats and to explore the changing rules of energy substances after death. Methods The levels of ATP, ADP and AMP in the gastrocnemius, myocardium and liver of rats at different time points after death were detected by high performance liquid chromatography (HPLC). The results showed that ATP in the gastrocnemius muscle decreased rapidly with the prolongation of death time and was undetectable or trace detected at 8h after death. The content of ATP in myocardium and liver tissue was greatly affected by the way of death. ADP can be detected at the entire detection time point, and the content of AMP changes irregularly. Conclusion The detection of adenosine triphosphate (ATP) by HPLC showed that the change of ATP content in the gastrocnemius, myocardium and liver tissue of rats in 24h correlated with the time of death, which could provide a new and objective reference for the inference of death time of forensic medicine.