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目的 明确鼻咽癌组织端粒酶各组分 (hTR、TP1、hTERT)基因表达及其与端粒酶活性关系。方法 采用RT PCR和PCR ELISA方法分别检测同一标本 ,包括鼻咽癌 (NPC)组织、慢性鼻咽炎(CINE)组织端粒酶各组分基因表达和端粒酶活性。结果 (1) 4 3例NPC组织中 ,38例 (88% )可检测到hTERTmRNA表达 ;16例CINE组织中均未能检测到hTERTmRNA表达 ,hTETRmRNA在NPC组织中表达显著高于CINE组织中hTERTmRNA的表达 (P <0 0 5 )。 (2 ) 4 3例NPC组织中 ,39例 (90 7% )表达hTR ,38例 (88% )表达TP1mRNA ;16例CINE组织中 ,14例 (87 5 % )分别表达hTR和TP1mRNA。hTR或TP1mRNA在NPC和CINE组织中表达比较差异无显著意义 (P >0 0 5 )。 (3)NPC组织端粒酶活性 (86 % )显著高于CINE组织端粒酶的活性 (0 % ) (P <0 0 5 )。 (4 )hTERTmRNA表达与端粒酶活性显著相关 (P <0 0 5 ) ;而hTR或TP1mRNA的表达与端粒酶活性无关 (P >0 0 5 )。 (5 )端粒酶各组分基因表达均与NPC临床分期和有无颈淋巴结转移无关 (P >0 0 5 )。结论 hTR和TP1mRNA在NPC和CINE组织中均高阳性率表达 ,并与端粒酶活性无关 ,hTERTmRNA仅在NPC组织中表达 ,与端粒酶活性呈高度一致性 ,提示hTERT在端粒酶活性调节中可能起决定性作用 ,对hTERTmRNA检测可以作为NPC?
Objective To investigate the expression of telomerase (hTR, TP1, hTERT) and its relationship with telomerase activity in nasopharyngeal carcinoma. Methods RT-PCR and PCR-ELISA were used to detect the gene expression and telomerase activity of telomerase components in the same specimen, including nasopharyngeal carcinoma (NPC) and chronic nasopharyngitis (CINE). Results (1) hTERT mRNA expression was detected in 38 cases (88%) of 43 NPC cases. The expression of hTERT mRNA was not detected in 16 cases of CINE tissues. The expression of hTETR mRNA in NPC tissues was significantly higher than that of hTERT mRNA in CINE tissues Expression (P <0 05). Thirty-nine cases (90.7%) expressed hTR and 38 cases (88%) expressed TP1mRNA. Of the 16 cases of CINE, 14 (87.5%) expressed hTR and TP1mRNA, respectively. The expression of hTR or TP1 mRNA in NPC and CINE tissues was not significantly different (P> 0.05). (3) The telomerase activity (86%) in NPC was significantly higher than that in CINE (0%) (P <0.05). (4) The expression of hTERT mRNA was significantly correlated with telomerase activity (P <0 05), but the expression of hTR or TP1 mRNA was not associated with telomerase activity (P 0 05). (5) The gene expression of each component of telomerase had no relation with the clinical stage of NPC and the presence or absence of cervical lymph node metastasis (P> 0.05). Conclusion The expression of hTR and TP1 mRNA in both NPC and CINE tissues is highly positive, and not related to telomerase activity. HTERT mRNA is only expressed in NPC tissues and highly consistent with the telomerase activity, suggesting that hTERT is regulated in telomerase activity May play a decisive role in hTERT mRNA detection can be used as NPC?