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1984、1985年的试验结果,明确了温度、紫外光、日光及缓冲液pH值等几种物理因素对山楂粉蝶核型多角体病毒Acr NPV感染力的影响.Acr NPV分别在40℃冰箱中保存14个月、28个月和在果园树干上保留14个月的Acr NPV虫尸,对幼虫的感染力无明显变化.Acr NPV存32℃恒温处理后,其感染力随时间延长而逐渐下降.紫外光对Acr NPV杀伤力较大,当紫外光照射240分钟后,Acr NPV完全丧失了对幼虫的感染力.果园试验表明Acr NPV悬液喷在果树叶正面和背面时,对幼虫的感染力有差异.Acr NPV对幼虫的感染力和磷酸缓冲液的PH值有关,当pH值为3.5或10.5时,感病幼虫发病慢,当pH值为7.2时,幼虫发病快.
1984 and 1985, the effects of several physical factors such as temperature, ultraviolet light, sunlight and buffer pH value on the infectivity of Acr NPV of hawthorns psittaci nucleopolyhedrovirus were determined.Acr NPV were stored in refrigerator at 40 ℃ Acr NPV larvae had no significant changes in larvae after 14 months, 28 months and 14 months in the trunk of the orchard .Acr NPV gradually decreased with prolonged incubation at 32 ℃. Acr NPV was more lethal to UV light than Acr NPV, and Acr NPV completely lost its infectivity to larvae after 240 minutes exposure to ultraviolet light. Orchard experiments showed that the infectivity of Acr NPV to larvae when sprayed on the front and back of the leaves There was a difference between the infectivity of Acr NPV and larvae pH value of phosphate buffer solution, when the pH value of 3.5 or 10.5, the susceptible larvae slow onset, when the pH value of 7.2 larvae fast onset.