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对引进的139份主要来自湖南省的大豆种质资源,苗期采用简单钙溶液法和全营养液法,以主根相对伸长率为评价指标,分析大豆对铝毒的耐性表现。结果表明,大豆对铝毒的耐性存在基因型差异。首先通过简单钙培养法筛选得到耐酸铝材料12份,之后通过全营养法进行验证获得耐酸铝大豆种质资源8份,即沅陵矮子早<甲>、长沙夏黄豆、常德中和青豆、凤凰青皮豆<乙>、十月青豆、人潮溪黄豆1、中作04563、中作05675。进一步以垦丰15、桂春8号为对照品种,采用土培法对获得的8份大豆耐性材料进行盆栽鉴定,以单株粒重、百粒重、叶绿素含量和株高作为耐铝毒性的评价指标,分析大豆对铝毒耐性的综合表现。遴选出1份耐性较强的材料,即人潮溪黄豆1,可供大豆耐铝毒性遗传育种研究利用。
The 139 soybean germplasm resources mainly from Hunan Province were introduced, the simple calcium solution method and the whole nutrient solution method were used in the seedling stage, and the relative elongation of the main root was taken as the evaluation index to analyze the tolerance of soybean to aluminum toxicity. The results showed that there was genotypic difference in the tolerance of soybean to aluminum toxicity. First of all, 12 samples of acid-proof aluminum were screened by simple calcium culture method, and then 8 samples of acid-proof aluminum soybean germplasm were obtained through the whole-nutrition method, that is, Yuanling dwarf Zijia> Changsha summer beans, Changde neutralized green beans, Green beans , October green beans, tide Creek 1, in 04563, made in 05675. Taking Kenfeng 15 and Guichun 8 as control varieties, eight cultivars of soybean tolerant material were identified by pot culture. The weight per plant, grain weight, chlorophyll content and plant height of the cultivars were used as aluminum tolerant Evaluation index, analyze the comprehensive performance of soybean tolerance to aluminum toxicity. Select a patience of strong material, namely tide Creek soybeans 1, available for soybean aluminum toxicity genetic breeding research and utilization.