目的:探讨回转器模拟失重对人脐静脉内皮细胞(HUVECs)形态、增殖和周期的影响。方法:以HUVECs为研究对象,采用回转器模拟失重,在倒置显微镜下观察模拟失重后细胞形态的变化,用细胞计数法测定细胞的增殖情况,用流式细胞仪(FCM)测定细胞周期。结果:模拟失重后,HUVECs多呈圆形,可见细胞重叠或聚集生长。回转器模拟失重后12 h、24 h和36 h,细胞周期明显阻滞在G0+G1期(P<0.05),而细胞计数则无明显改变。结论:模拟失重可使HUVECs的形态发生一定改变,使HUVECs的细胞周期阻滞。 Objective: To investigate the effects of rotameter simulated weightlessness on the morphology, proliferation and cycle of human umbilical vein endothelial cells (HUVECs). Methods: The HUVECs were used as the research objects. The rotator was used to simulate the weightlessness. The morphological changes of the cells were observed under an inverted microscope. The cell proliferation was measured by the cell counting method. The cell cycle was determined by flow cytometry (FCM). Results: After simulated weightlessness, HUVECs were mostly round, showing cell overlapping or aggregated growth. At 12 h, 24 h and 36 h after revolver simulated weightlessness, the cell cycle was significantly arrested in G0 + G1 phase (P <0.05), while the cell count did not change significantly. Conclusion: Simulated weightlessness can change the morphology of HUVECs and block the cell cycle of HUVECs.