论文部分内容阅读
目的检测和分析组蛋白去甲基化酶含十字形结构域蛋白3(JMJD3)在胶质瘤及瘤旁脑组织中的表达。方法用组织芯片筛选出JMJD3表达阳性的胶质瘤组织,选取50例相关胶质瘤患者的石蜡标本,采用免疫组化SP法检测JMJD3在相关胶质瘤组织中的表达与定位以及与瘤旁组织中表达的差异,分析JMJD3与胶质瘤的相关性。结果通过组织芯片筛选,发现JMJD3在正常少突胶质细胞中有表达。在50例胶质瘤患者的石蜡标本中,15例标本肿瘤组织为阳性,35例瘤旁组织为阳性。结论与瘤旁脑组织相比,胶质瘤JMJD3的表达降低。
Objective To detect and analyze the expression of histone demethylase containing cross-shaped domain protein 3 (JMJD3) in gliomas and para-tumor tissues. Methods Tissue microarray was used to detect the expression of JMJD3 in glioma tissues. Fifty paraffin-embedded specimens of glioma were collected. Immunohistochemical SP method was used to detect the expression and localization of JMJD3 in glioma tissues. Differences in the expression of tissue, analysis of JMJD3 and glioma correlation. Results By tissue chip screening, JMJD3 was found to be expressed in normal oligodendrocytes. In the paraffin specimens of 50 patients with glioma, 15 cases of specimens of tumor tissue was positive, 35 cases of para-tumor tissue was positive. Conclusions The expression of JMJD3 in glioma is lower than that in tumor-adjacent brain.