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目的:建立HPLC法同时测定杞蓉片中五味子醇甲、欧前胡素和蛇床子素含量。方法:采用Diamonsil C18(200mm×4.6mm,5μm)色谱柱,以甲醇-水(65∶35)为流动相;流速1.0mL·min-1,柱温35℃;检测波长250nm。结果:五味子醇甲、欧前胡素和蛇床子素进样浓度分别在4～40μg·mL-1(r=0.9997),2.46～24.6μg·mL-1(r=0.9999),16.5～165μg·mL-1(r=0.9997)范围内,与峰面积呈良好的线性关系;平均回收率(n=9)分别为102.3%,101.5%,102.0%。结论:本方法简便、准确,专属性强,为杞蓉片的质量控制提供了依据。
Objective: To establish a HPLC method for the simultaneous determination of schisandrin, imperatorin and osthole in Qirong tablets. METHODS: Diamonsil C18 (200mm × 4.6mm, 5μm) column was used with methanol-water (65:35) as mobile phase. The flow rate was 1.0mL · min-1 and the column temperature was 35 ℃ .The detection wavelength was 250nm. Results: The injection concentrations of Schisandrin, Imperatorin and Osthole were 4 ~ 40μg · mL -1 (r = 0.9997), 2.46 ~ 24.6μg · mL -1 (r = 0.9999), 16.5 ~ 165μg · mL-1 (r = 0.9997). The average recoveries (n = 9) were 102.3%, 101.5% and 102.0%, respectively. Conclusion: The method is simple, accurate and specific and provides the basis for the quality control of Qirong Tablets.