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目的建立非CODIS系统miniSTR以及Amelogenin基因座的荧光复合扩增体系。方法筛选8个多态性高的非CODIS系统miniSTR基因座(D20S1082、D6S474、D12ATA63、D9S1122、D2S1776、D1S1627、D3S4529、D2S441),并结合Amelogenin基因座设计荧光标记引物,优化反应条件,建立复合扩增体系。应用该体系对204份广州地区汉族血样,30个家系样本,及30份降解检材进行检测。结果建立的荧光标记8个miniSTR及Amelogenin复合扩增体系分型结果明确,稳定性好,且所有片段长度均少于200bp,提高了降解检材的分型成功率。在广州汉族人群的累积个人识别率为0.99999993,累积非父排除率为0.992287。结论构建的miniSTR荧光复合扩增体系,操作简便,分型准确,重复性好,对降解检材有效,易于在法医实验室推广应用,可对现有试剂盒起补充作用。
Objective To establish a fluorescence multiplex amplification system for miniSTR and Amelogenin loci of non-CODIS system. Methods The miniSTR loci (D20S1082, D6S474, D12ATA63, D9S1122, D2S1776, D1S1627, D3S4529, D2S441) of 8 non-CODIS system with high polymorphism were screened. Fluorescently labeled primers were designed according to the Amelogenin locus to optimize the reaction conditions. Increased system. Using this system, 204 samples of Han nationality, 30 pedigrees and 30 samples of degradation in Guangzhou were detected. Results The fluorescent labeled miniSTR and Amelogenin composite amplification system established clear typing results with good stability and all the fragments were less than 200 bp in length, which improved the typing success rate of degraded samples. The cumulative personal recognition rate in Guangzhou Han population was 0.99999993, and the cumulative non-parent exclusion rate was 0.992287. Conclusion The constructed miniSTR fluorescence multiplex amplification system has the advantages of simple operation, accurate typing, good repeatability, effective degradation samples and easy application in forensic laboratories, which can supplement existing kits.