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目的为探讨X线照射新生大鼠后对小脑皮质浦氏细胞层排列的影响。方法将新生一日(P1)大鼠经X线全身照射(剂量为1.5Gy),照射后3周取小脑用于Nissl染色及免疫组化染色分析。Calbindin-D28k染色和GFAP染色标记浦氏细胞及在小脑发生过程中起重要作用的贝格曼神经胶质细胞。结果 X线照射的新生大鼠3周后可见小脑变小,部分小脑叶排列紊乱。与对照组相比,Calbindin D-28k阳性细胞未能形成单层排列,部分细胞侵入颗粒细胞层,且树突发育不良。同时可见GFAP阳性细胞纤维未能通过分子层而达软膜下。结论 X线照射新生大鼠后,贝格曼神经胶质细胞纤维的排列紊乱导致小脑皮质浦氏细胞层排列的异常。
Objective To investigate the effect of X-ray irradiation on the arrangement of the layer of Putraleis in cerebellar cortex. Methods Whole body X-ray irradiation (1.5 Gy) was performed on neonatal day (P1) rats. The cerebellum was taken for Nissl staining and immunohistochemical staining three weeks after irradiation. Calbindin-D28k staining and GFAP staining marked Pucci cells and plays an important role in the process of cerebellar Berger glia. Results X-ray irradiation of neonatal rats after 3 weeks showed a smaller cerebellum, part of the cerebellar lobes disorder. Compared with the control group, Calbindin D-28k positive cells failed to form a monolayer arrangement, some of the cells invaded the granular cell layer, and dendritic dysplasia. At the same time can be seen GFAP positive cells failed to reach the submucosa through the molecular layer. Conclusion X-ray irradiation in neonatal rats, Bergemann glial cells arranged in disorder fibers lead to abnormal cerebellar cortex Pu’s cell layer arrangement.