活血化瘀方有较好的抗肝纤维化作用 ,为探讨活血化瘀方影响肝纤维化发生、发展的作用机制 ,我们采用MTT法检测活血化瘀方药物血清对HSC增殖的影响 ,免疫细胞化学法研究活血化瘀方药理血清对HSC表达α SMA的影响。结果表明 ,药物血清组MTT法OD值、α SMA阳性染色吸光度分别为对照组的 58 8%、51 2 % (P <0 0 1)。活血化瘀方药物血清与γ IFN有相似作用 ,能够通过抑制HSC增殖、α SMA的表达抑制体外培养的HSC的激活。 Huoxue Huayu Decoction has better anti-hepatic fibrosis effect. To investigate the mechanism of Huoxuehuayu Decoction affecting the occurrence and development of hepatic fibrosis, we used MTT assay to detect the effect of serum of Huoxuehuayu Decoction on proliferation of HSCs. Study on the Effect of Pharmacological Serum of Activating Blood Circulation to Remove Blood Stasis on α - SMA Expression of HSC by Chemical Method . The results showed that the MTT OD value and the α SMA positive staining absorbance in the drug serum group were 58 8% and 51 2% of the control group, respectively (P < 0 01). Huoxuehuayu prescription drug serum and γ IFN have similar effects and can inhibit the activation of HSC cultured in vitro by inhibiting HSC proliferation and α SMA expression.