论文部分内容阅读
目的 探讨脂肪酸合酶抑制剂———浅蓝菌素对K5 6 2白血病细胞增殖的影响及机制 ,并观察浅蓝菌素对人皮肤成纤维细胞增殖的影响。方法 应用MTT法观察浅蓝菌素对K5 6 2白血病细胞、人皮肤成纤维细胞增殖的抑制率 ;应用流式细胞术、DNA凝胶电泳进行凋亡的检测和观察。结果 在 10 - 9～ 10 - 5 mol L浓度下 ,浅蓝菌素对K5 6 2白血病细胞的增殖有显著的抑制作用并呈剂量 效应关系 ;而人皮肤成纤维细胞在浅蓝菌素 (10 - 9～ 10 - 5 mol L)作用下 ,抑制率均 <30 % ;K5 6 2白血病细胞在浅蓝菌素 (6 0 μg ml,12h)作用下 ,DNA凝胶电泳出现梯状条带。 结论 ①脂肪酸合酶抑制剂———浅蓝菌素显著地阻遏K5 6 2白血病细胞的增殖且不影响人皮肤成纤维细胞的增殖 ;②浅蓝菌素对K5 6 2细胞的细胞毒作用可能通过凋亡而诱导 ;③脂肪酸合酶是一个潜在的抗白血病靶位
Objective To investigate the effect and mechanism of cerulenin, an inhibitor of fatty acid synthase, on the proliferation of K562 cells, and to observe the effect of cerulenin on the proliferation of human skin fibroblasts. Methods MTT assay was used to observe the inhibition rate of cerulenin on proliferation of K562 cells and human skin fibroblasts. Flow cytometry and DNA gel electrophoresis were used to detect and observe apoptosis. Results At the concentration of 10 -9 to 10 -5 mol L, ceruletin had a significant inhibitory effect on the proliferation of K562 cells and showed a dose-effect relationship, while human skin fibroblasts were exposed to cerulenin (10 The inhibitory rate was less than 30% under the condition of 9～10 -5 mol L). The ladder-like bands appeared in DNA gel electrophoresis under the action of cerulenin (60 μg ml, 12 h) in K562 cells. Conclusion 1 The cerulenin, an inhibitor of fatty acid synthase, significantly inhibited the proliferation of K562 cells without affecting the proliferation of human skin fibroblasts. 2 Cytotoxic effect of cerulenin on K562 cells may be possible. Induced by apoptosis; 3 fatty acid synthase is a potential anti-leukemia target