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目的探讨晚期乳腺癌患者的癌细胞对多西紫杉醇、长春新碱、阿霉素、顺铂等化疗药物的敏感性。方法收集2007年至2012年晚期乳腺癌患者的乳腺癌组织共58例。在无菌条件下剪成两部分,一部分采用胶原酶消化法取得乳腺癌原代细胞并进行体外原代培养,研究多西紫杉醇、长春新碱、阿霉素、顺铂在0.2、0.5、1.0、1.5、2.0PPC 5个浓度下对乳腺癌原代细胞生长的影响,用MTT法检测细胞生长抑制率。另一部分制成石蜡切片,用SP法检测多药耐药基因(MDR1)、耐药相关蛋白(MRP)的表达情况。结果①与MDR1阳性的乳腺癌相比,多西紫杉醇、长春新碱、阿霉素对MDR1阴性的乳腺癌细胞的抑制作用强(P<0.05);而顺铂的抑制作用无明显差异(P>0.05)。②与MRP阴性的乳腺癌相比,四种药物中只有多西紫杉醇对MRP阳性的乳腺癌细胞的抑制作用无明显差异(P>0.05)。结论顺铂对MDR1阳性的乳腺细胞的敏感性较好,多西紫杉醇对MRP阳性的乳腺癌细胞的敏感性较好。
Objective To investigate the sensitivity of cancer cells to docetaxel, vincristine, doxorubicin, cisplatin and other chemotherapy drugs in patients with advanced breast cancer. Methods A total of 58 cases of breast cancer from 2007 to 2012 were collected. Cut into two parts under aseptic conditions, a part of collagenase digestion obtained primary breast cancer cells and primary culture in vitro study docetaxel, vincristine, doxorubicin, cisplatin in 0.2,0.5,1.0 , 1.5,2.0PPC 5 concentrations of breast cancer primary cell growth, MTT assay cell growth inhibition rate. The other part was made into paraffin sections, and the expression of multidrug resistance gene (MDR1) and drug resistance associated protein (MRP) was detected by SP method. Results ① Compared with MDR1-positive breast cancer, docetaxel, vincristine and doxorubicin had a stronger inhibitory effect on MDR1-negative breast cancer cells (P <0.05), while the inhibitory effect of cisplatin was not significantly different (P > 0.05). ② Compared with MRP-negative breast cancer, only docetaxel in the four drugs showed no significant difference in MRP-positive breast cancer cells (P> 0.05). Conclusion Cisplatin is more sensitive to MDR1-positive breast cancer cells and docetaxel is more sensitive to MRP-positive breast cancer cells.