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自从氯丙嗦(CPZ)被用作镇静剂以来,它的光毒性就为人们熟知,但光毒性的确切机制还未完全清楚。由于在某些光毒反应中发现补体活化,因此作者通过下述方法评价CPZ光毒反应中补体系统的作用:(1)CPZ加入正常健康成人血清中,以不同剂量UVA照射,照射后测定血清总补体溶血活性(CH_(50))。结果随着照射剂量的加大CH_(50)进行性下降。(2)照射后血清应用交叉免疫电泳测定C_3的转换,发现C_3从β_1转变为β_1A。(3)雌性白化豚鼠先经腹腔内注射300u/kg的眼镜蛇毒素进
Its phototoxicity has been well known since chlorpromazine (CPZ) has been used as a sedative, but the exact mechanism of phototoxicity is not fully understood. Since complement activation was found in some phototoxic reactions, the authors evaluated the effects of complement systems in CPZ phototoxic reactions by the following methods: (1) CPZ was added to normal healthy adult serum and irradiated with different doses of UVA, after which the serum was measured Total complement hemolysis activity (CH_ (50)). Results As the dose of radiation increased, CH_ (50) decreased progressively. (2) After the irradiation, the C_3 translocation was measured by the cross-immunoelectrophoresis in the serum and C_3 was changed from β_1 to β_1A. (3) Female albino guinea pigs were injected intraperitoneally with 300u / kg of cobra venom