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目的:研究釉基质蛋白对双重热酸蚀及碱热处理纯钛表面仿生矿化沉积磷灰石层的影响。方法:提取猪未萌恒牙胚表面EMPs,用SDS-PAGE电泳进行验证。纯钛片经抛光清洗,分别进行双重热酸蚀和碱热处理,放入含EMPs(150μg/mL)的改良模拟体液(m-SBF)中浸泡7d,对照组为不含EMPs的m-SBF。扫描电镜观察试件表面形貌,X射线能谱,X射线衍射及傅里叶红外光谱等分析其元素成分及晶相结构。结果:酸蚀组经仿生矿化,对照组试件表面无沉积物生成,实验组加入EMPs,钛片表面有一定量的沉积物生成,能谱分析显示主要由Ca、P、O和C等元素组成。碱热处理组经仿生矿化,对照组与实验组试件表面均有沉积层形成,但后者有较多直径约为300～600nm的孔隙生成,元素组成主要为Ca、P、O和C,X射线衍射及傅里叶红外光谱分析显示沉积物为碳羟基磷灰石。结论:碱热处理纯钛表面有利于磷灰石层的形成,加入EMPs能促进磷灰石层的形成并改变其形貌。
OBJECTIVE: To study the effect of enamel matrix protein on double thermal etching and alkali heat treatment of pure titanium surface bionic mineralized apatite layer. Methods: The surface EMPs of porcine immature embryos were extracted and verified by SDS-PAGE electrophoresis. Pure titanium pieces were polished and cleaned, double thermal etching and alkali treatment were respectively carried out, immersed in modified mimic body fluid (m-SBF) containing EMPs (150μg / mL) for 7 days, and control group were m-SBF without EMPs. The surface morphology, X-ray energy spectrum, X-ray diffraction and Fourier transform infrared spectroscopy (FTIR) were used to analyze the elemental composition and crystal structure of the sample. Results: The acid etching group was bionic and mineralized, and the control group had no sediment on the surface of the specimen. EMPs were added to the experimental group and a certain amount of sediment was formed on the surface of the titanium film. The energy spectrum analysis showed that Ca, P, O and C composition. Alkali heat treatment group bionic mineralization, the control group and the experimental group were deposited on the surface of the specimen layer, but the latter is more about the diameter of about 300 ~ 600nm pore generation, the main elemental composition of Ca, P, O and C, X-ray diffraction and Fourier transform infrared spectroscopy showed that the deposit was carbon-hydroxyapatite. CONCLUSION: Alkali heat treatment of pure titanium favors the formation of apatite layer. Adding EMPs can promote the formation of apatite layer and change its morphology.