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目的分离纯化驱虫斑鸠菊中紫铆素、紫铆花素,初步探讨这两种化合物对人A375黑素瘤细胞增殖及黑素合成作用的影响。方法采用硅胶、Sephadex LH-20反复柱层析分离纯化紫铆素、紫铆花素;分别采用MTT法、酶学法及NaOH法测定这两种化合物对人A375黑素瘤细胞的增殖、酪氨酸酶活性及黑素合成的影响。结果分离得到化合物紫铆素、紫铆花素,在0.50~10.00μg·mL-1内紫铆素、紫铆花素促进A375细胞增殖,且紫铆花素促增殖作用强于紫铆素(P<0.05);在0.10~1.00μg·mL-1内两种化合物均上调酪氨酸酶活性;在0.50~5.00μg·mL-1内,紫铆花素促进黑素合成,紫铆素抑制黑素合成。结论紫铆素、紫铆花素可能是驱虫斑鸠菊的药效成分;紫铆花素促进A375细胞的增殖,且使A375细胞黑素合成增加,紫铆素轻度抑制黑素合成。
OBJECTIVE To isolate and purify verotinin and vinca anthocyanin from Vernonia lutea, and to investigate the effects of these two compounds on the proliferation and melanogenesis of human A375 melanoma cells. Methods Purification of vinorelbine and Vainin by silica gel and Sephadex LH-20 column chromatography were used to determine the proliferation of human A375 melanoma cells by MTT, enzymatic and NaOH methods respectively. The Effect of Enzyme Activity and Melanin Synthesis. The results showed that the compounds privastin and vinca cinobufacient were isolated and primed with Vitexin and Vaseline to promote the proliferation of A375 cells in 0.50 ~ 10.00μg · mL-1, and the proliferation effect of Vistula is stronger than that of Vitexin P <0.05). In the range of 0.10 ~ 1.00μg · mL-1, both compounds increased tyrosinase activity; within 0.50 ~ 5.00μg · mL-1, Melanin synthesis. Conclusion Vitexin and Varisin may be the active ingredients of Vernonia lutea. Vishin can promote the proliferation of A375 cells and increase the melanin synthesis of A375 cells. Violetin slightly inhibits melanin synthesis.