论文部分内容阅读
目的 观察大鼠胰腺缺血／再灌注损伤对胰腺组织氧自由基（ＭＤＡ）、胰腺组织钙、胰腺细胞凋亡等的变化及异搏定对缺血／ 再灌注损伤胰腺的保护作用。方法 采用钳闭大鼠腹腔干、肠系膜上动脉制备胰腺缺血／再灌注损伤模型。取Ｗｉｓｔａｒ 大鼠６３ 只，随机分为假手术组、缺血／再灌注组、缺血／ 再灌注异搏定保护组。结果 缺血／ 再灌注异搏定保护组大鼠胰腺组织ＭＤＡ、组织钙、胰腺细胞凋亡明显较缺血／ 再灌注组低，病理改变轻。缺血／ 再灌注组胰腺细胞凋亡与ＭＤＡ、组织钙存在相关性。结论 异搏定降低胰腺组织ＭＤＡ和组织钙含量，抑制细胞凋亡，保护缺血／ 再灌注损伤的胰腺；胰腺缺血／ 再灌注损伤中存在细胞凋亡机制的参与；细胞凋亡存在ＭＤＡ和组织钙超载的参与。
Objective To observe the effects of Isoptin on the pancreas in the pancreas of pancreas during ischemia / reperfusion (IR) and pancreatic islet (MDA), pancreatic calcium and pancreatic cell apoptosis in rats. Methods Rat models of pancreatic ischmia / reperfusion injury were established by clamping the celiac trunk and superior mesenteric artery. A total of 63 Wistar rats were randomly divided into sham operation group, ischemia / reperfusion group and Ischemia / reperfusion Weixifen protection group. Results Ischemia / reperfusion Weisuoding protective group rats pancreatic tissue MDA, tissue calcium, pancreatic apoptosis was significantly lower than ischemia / reperfusion group, pathological changes in light. There was a correlation between apoptosis of pancreatic cells and MDA and tissue calcium in ischemia / reperfusion group. Conclusion Verapamil may reduce the pancreatic tissue MDA and tissue calcium content, inhibit apoptosis and protect the pancreas from ischemia / reperfusion injury. Pancreatic ischemia / reperfusion injury may play a role in the mechanism of apoptosis. Tissue calcium overload involved.